Process for the preparation of the cholecystokinin-pancreozymin octapeptide amide sulfate ester
US4102878A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 28, 1977 |
| Grant date | Jul 25, 1978 |
| Priority date | — |
| Expiry date | Apr 28, 1997 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P20/55
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for the preparation of L-aspartyl-(O-sulfato-L-tyrosyl)-L-methionyl-glycyl-L-tryptophyl-L-methion yl-L-aspartyl-L-phenylalanine amide, which comprises treating the protected octapeptide amide of the general formula (II) ##STR1## wherein X is BOC, Cbz or Ddz, PA1 Y is O.sup.t Bu, OBzl or OH and PA1 W is O.sup.t Bu, OBzl or OH, in the presence of an ether with an excess of the complex compound of sulfur trioxide and a tertiary amine containing at least one alkyl group, converting the obtained sulfate ester of the protected octapeptide amide into an alkali metal salt and simultaneously removing the formyl group from the tryptophan unit by reacting the sulfate ester with an alkali metal hydroxide, and then splitting off the remaining protective groups by acidolysis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.