Cell specific, variable density, polymer microspheres
US4105598A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 3, 1977 |
| Grant date | Aug 8, 1978 |
| Priority date | — |
| Expiry date | Feb 3, 1997 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T428/2982
- WIPO fieldMacromolecular chemistry, polymers
- WIPO sectorChemistry
Abstract
Biocompatible polymeric microspheres having an average diameter below about 3 microns and having a density at least 15% greater or lesser than organic cells and having covalent binding sites are provided in accordance with this invention. The microspheres are obtained by copolymerizing a hydroxy or amine substituted acrylic monomer such as hydroxyethylmethacrylate with a light or dense comonomer such as a fluoromonomer. A lectin or antibody is bound to the hydroxy or amine site of the bead to provide cell specificity. When added to a cell suspension the marked bead will specifically label the cell membrane by binding to specific receptor sites thereon. The labelled membrane can then be separated by density gradient centrifugation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.