Method for rapid analysis of hemoglobin variants by high speed liquid chromatography
US4108603A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 18, 1977 |
| Grant date | Aug 22, 1978 |
| Priority date | — |
| Expiry date | May 18, 1997 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N30/96
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for analysis of hemoglobin variants by high speed liquid chromatography and, more particularly, by ion-exchange chromatography. Through the use of high flow rates and pressures, the hemoglobin variants can be rapidly forced through the separation column to thus enable rapid analysis. A bonded phase inorganic support column is utilized for separation of the hemoglobin variants with the profiles thereof then being obtained. The bonded phase support preferably includes ion-exchange groups bonded through a carbohydrate intermediate to controlled porosity glass. A pair of different columns may be utilized to separate the hemoglobin variants with one of the columns being an anion-exchanger, and more particularly, a diethylaminoethanol glycophase, controlled porosity glass column, with the other column being a cation-exchanger, and more particularly, a carboxymethyl glycophase, controlled porosity glass column. The hemoglobin profiles obtained from such a pair of columns are different and the combination of chromatographic data obtained identify the particular hemoglobin variants and can thus be utilized for diagnosis purposes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.