Vasoactive lung polypeptides

US4113711A · kind A · utility

Inventors

• Sami I. Said · Dallas, US
• Viktor Mutt · Solna, SE

Key dates

• Filing date: Jan 19, 1976
• Grant date: Sep 12, 1978
• Expiry date: Jan 19, 1996

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/848
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Vasoactive polypeptide fractions having biological activity isolated in purified form from animal lungs induce peripheral vasodilation and systemic hypotension. One of the peptides exhibits the property of relaxing isolated non-vascular smooth muscle organs while the other peptide contracted these tissues. The peptide fractions are obtained from the lungs of animals by procedures involving boiling and mincing the lung, extracting the peptides into acetic acid, adsorbing by alginic acid, eluting with HCl and salting out, extracting into alcohol, again adsorbed with alginic acid, fractionating by gel chromatography, e.g. on Sephadex G-25, and by ion exchange chromatography, e.g. on carboxymethyl cellulose, followed by further ion exchange chromatography and, optionally, countercurrent distribution.