Process for fixing proteins onto carriers

US4119589A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Jurgen Horn · Egelsbach, DE
• Hans-Georg Batz · Tutzing, DE
• Dieter Jaworek

Key dates

• Filing date: Jan 18, 1977
• Grant date: Oct 10, 1978
• Expiry date: Jan 18, 1997

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/816
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Biologically active proteins are immobilized onto a carrier by reacting a compound containing at least two secondary amino groups at a temperature of from -80.degree. to +50.degree. C with a chlorination agent with the conversion of the secondary amino groups into iminochloride groups of the formula ##STR1## in which R and R.sub.1, which can be the same or different, are aliphatic, aromatic or aliphatic-aromatic radicals, and reacting the carrier material product thus obtained PA1 (a) directly with a biologically-active protein, in an aqueous solvent, or PA1 (b) with a bifunctional compound containing at least one primary amino group, optionally in an aqueous or organic solvent, and reacting the product thereby obtained containing groups of the formula ##STR2## in which R.sub.2 is an alkylene radical, an .alpha.,.omega.-dioxyalkylene radical or a dicarboxylic acid amide group containing 2 to 8 carbon atoms and X is a primary amino group or a function capable of protein binding either PA1 (i) directly with a protein or PA1 (ii) first with at least one bifunctional or polyfunctional protein reagent and thereafter with a protein or with a reaction product of a protein with the protein…