Heparin purification method

US4119774A · kind A · utility

Assignee

• AB Kabi · SE

Inventors

• Lars Andersson · Knivsta, SE
• Erik Holmer · Stockholm, SE

Key dates

• Filing date: Mar 2, 1977
• Grant date: Oct 10, 1978
• Expiry date: Mar 2, 1997

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C08B37/0075
• WIPO field: Macromolecular chemistry, polymers
• WIPO sector: Chemistry

Abstract

Crude clinically used heparin preparations having a low specific activity, for example, about 130 heparin units per milligram are purified by contacting such liquid crude heparin preparations with a gel matrix complexed with (i.e. having linked to it) antithrombin or inter-alpha-trypsin inhibitor as a heparin-binding plasma protein, and then desorbing or eluting the heparin from the antithrombin or inter-alpha-trypsin inhibitor complexed gel matrix as with an applicable buffer, removing any salt in the solution with the heparin by gel filtration and then drying the heparin as by lyophilization.