Process for mounting tissue sections with an U.V. light curable mounting medium
US4120991A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 10, 1976 |
| Grant date | Oct 17, 1978 |
| Priority date | — |
| Expiry date | Dec 10, 1996 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N1/312
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Apparatus and method for permanently and protectively mounting "wet" thin sections of biological specimens on microscope slides, wherein each specimen is initially bathed in a low-volatile solvent containing a low concentration of first polymerizable material. Such solvent is allowed to evaporate, whereby the liquid polymerizable material permeates and protects the specimen. Following solvent evaporation, a second polymerizable material is layered over the specimen. Preferably, the first and second polymerizable materials comprise a mixture of low-volatility, low-viscosity, liquid acrylic reactomers and a U.V. light-sensitive catalyst system. A conventional cover slip or other planar transparent member can be positioned over the polymerizable materials, and the same are polymerized by exposure to U.V. radiation. The first and second now-polymerized materials encapsulate the specimen on the microscope slide, become integral and completely hardened and are fully devoid of any solvent. The microscope slide is immediately available for examination.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.