Specific binding assay with an enzyme modulator as a labeling substance
US4134792A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 6, 1976 |
| Grant date | Jan 16, 1979 |
| Priority date | — |
| Expiry date | Dec 6, 1996 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/817
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A specific binding assay method employing, as a labeling substance, a reversibly binding enzyme modulator for the detection of a ligand in a liquid medium. The method follows conventional specific binding assay techniques of either the homogeneous or heterogeneous type wherein the liquid medium to be assayed is combined with reagent means that includes a labeled conjugate to form a binding reaction system having a bound-species and a free-species of the conjugate. The amount of conjugate resulting in the bound-species or the free-species is a function of the amount of ligand present in the liquid medium assayed. In the present invention, the labeled conjugate comprises a reversibly binding enzyme modulator covalently linked to a binding component of the binding reaction system. The distribution of the conjugate between the bound-species and the free-species is determined by addition of an enzyme whose activity is affected, either decreased or increased, by said modulator and measuring the resulting activity of the enzyme. The enzyme modulator may be a conventional enzyme inhibitor, preferably of the coompetitive type, or an allosteric effector.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.