Protein product, a process for preparation, and application of said product
US4137222A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 11, 1975 |
| Grant date | Jan 30, 1979 |
| Priority date | — |
| Expiry date | Apr 11, 1995 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/851
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A gamma globulin-rich fraction from conventional ethanol fractionation of whole placenta is treated PA1 (i) with 17% ethanol at pH 5.1 (itself a conventional step in the fractionation) PA1 (ii) the precipitate is diluted with water and dialysed to remove alcohol, then LEDA (2-ethoxy-6, 9-diaminoacridine lactate) is added and the precipitate preferably at pH 7.7 .+-. 0.5 discarded PA1 (iii) ammonium sulphate at approximately semi-saturation is added and the precipitate collected. This precipitate contains a proteolytic enzyme (plasmin), assaged by caseinolysis and useful for removal of anti-complement from gamma globulin preparations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.