Cholesteroloxidase and method for its production from microorganisms

US4144129A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Wolfgang Gruber · Bischofswerda, DE
• Hans U. Bergmeyer
• Michael Nelbock-Hochstetter · Tutzing, DE
• Klaus Beaucamp · Tutzing, DE
• Gunter Holz
• Johanna Gramsall · Tutzing, DE
• Gunter Lang · Wernau, DE

Key dates

• Filing date: May 15, 1973
• Grant date: Mar 13, 1979
• Expiry date: May 15, 1993

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/872
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A new enzyme, cholesteroloxidase, is prepared by recovering same from microorganisms, preferably by washing a cholesterol-converting microorganism with butanol/water, disintegrating the microorganism culture and extracting same with a buffer solution containing a non-ionic surface-active agent, and isolating cholesterol oxidase from the extract.