Differential counting of leukocytes and other cells
US4146604A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jan 30, 1974 |
| Grant date | Mar 27, 1979 |
| Priority date | — |
| Expiry date | Jan 30, 1994 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/80
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A novel composition of matter, process and apparatus is provided for distinguishing and absolutely counting leukocytes, erythrocytes, reticulocytes, and other cells as well as differently counting and classifying the different types of leukocytes. The composition of matter comprises a plurality of compatible dyes for uniquely distinguishing leukocytes, erythrocytes, reticulocytes, and other cells randomly distributed in a medium. At least one of the dyes imparts a characteristic fluorescence to all leukocytes stained therewith. The nature of the dye composition is such that when the stained leukocytes are irradiated with radiation having the characteristic absorption wavelengths of each dye, the relative intensities of the light emitted and/or transmitted by each leukocyte type defines that particular leukocyte type. Apparatus is therefore provided for irradiating the medium with the characteristic radiation and measuring the radiation emitted by the irradiated leukocytes in each of their characteristic wavelength regions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.