Preparation of antiserum for quantitative determination of X and Y degradation products of fibrin and fibrinogen

US4147765A · kind A · utility

Assignee

• Biotest-Serum-Institut GmbH

Inventors

• Wolfgang Stephan · Heidelberg, DE
• Ronald Kotitschke · Dreieich, DE

Key dates

• Filing date: Jul 16, 1976
• Grant date: Apr 3, 1979
• Expiry date: Jul 16, 1996

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/83
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

An antiserum for the quantitative determination of X and Y degradation products of fibrin and fibrinogen is produced by administering the purified degradation products to an experimental animal thereby to immunize said animal, drawing serum from said animal, contacting said serum with a plasma and separating the resultant precipitate, thereby to leave a supernatant liquid which constitutes said antiserum. The plasma may be a heparin, citrate, oxalate, ethylenediaminetetracetate or glutaraldehyde-treated plasma and as many as 12 or 15 precipitations may be made with the animal antiserum. The antiserum may be fractionated at any stage by adsorption on Sephadex modified dextran.