Composition and method for the determination of ascorbic acid

US4152116A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Ulfert Deneke · Fürth, DE
• Gerhard Michal · Tutzing, DE
• Klaus Beauchamp · Tutzing, DE

Key dates

• Filing date: Jun 28, 1978
• Grant date: May 1, 1979
• Expiry date: Jun 28, 1998

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/14
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Ascorbic acid or dehydroascorbic acid is determined by reaction with a tetrazolium salt selected from 3-(4,5-dimethyl-thiazolyl-2)-2,5-diphenyl-tetrazolium bromide, nitrotetrazolium blue, tetrazolium blue chloride and distyryl-nitro blue tetrazolium chloride, in the presence of phenazine methosulphate and a detergent, at a weakly acidic pH value, and evaluating the coloration obtained as a measure of the initial acid content. The method is particularly efficacious in substantially eliminating disturbance of the reaction by foreign substances.