Method and apparatus for the continuous determination of the concentration of an enzyme substrate

US4153513A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Hermann Edelmann · Tutzing, DE
• Peter Kraft · Kassel, DE
• Karlheinz Mann · Weilheim, DE
• Peter Schuler · Karlsruhe, DE
• Alexander Hagen · Tutzing, DE

Key dates

• Filing date: Aug 19, 1977
• Grant date: May 8, 1979
• Expiry date: Aug 19, 1997

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N27/3271
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Enzyme substrates in aqueous liquids are continuously measured by introducing a sample containing the substrate to be determined into a current of buffer solution, reacting the substrate on an immobilized enzyme and measuring, in a measurement chamber, a physical change in the solution brought about by the reaction, wherein a current of buffer solution containing the sample to be determined is passed alternatingly (i) directly through the measurement chamber with the production of a reference signal and (ii) first over the immobilized enzyme and thereafter through the measurement chamber with the production of a measurement signal, and relating the reference signal to the measurement signal to indicate the enzyme substrate initially present.