Method for cryopreservation of multicellular organisms
US4155331A · kind A · utility
Inventors
Key dates
| Filing date | Apr 1, 1977 |
| Grant date | May 22, 1979 |
| Priority date | — |
| Expiry date | Apr 1, 1997 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A40/81
- WIPO fieldBasic materials chemistry
- WIPO sectorChemistry
Abstract
A method for the cryopreservation of multicellular organisms characterized by rapid cooling and warming rates, exceeding 100.degree. C. per minute, to achieve 80% survival after storage in the frozen state. The method involves exposing multicellular organisms to a cryoprotectant medium, freezing the organisms and medium by cooling at a rate greater than 100.degree. C. per minute, holding the organisms and medium at a point below 0.degree. C. for a period of time, and cooling the organisms and the medium at a rate greater than 100.degree. C. per minute to an appropriate storage temperature depending upon the desired length of the storage period. The method is more specifically described with regard to aquatic organisms, and particularly described with regard to the cryopreservation of immature marine crustaceans.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.