Flying spot fluoro-meter for oxidized flavoprotein and reduced pyridine nucleotide
US4162405A · kind A · utility
Inventors
Key dates
| Filing date | May 23, 1978 |
| Grant date | Jul 24, 1979 |
| Priority date | — |
| Expiry date | May 23, 1998 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N21/6402
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method and apparatus for measuring the heterogeneity of oxygen delivery to perfused and in situ organs. A laser is employed as a flying spot scanning excitation fluorometer source which provides excitation for either oxidized flavoprotein of the mitochondrial space or reduced nucleotide of mitochondrial and cytosolic spaces. Emission from the two fluorochromes is acquired at 10.sup.4 to 10.sup.5 data points per sec. and histograms of the fluorescence intensity versus the number of occurrences of that intensity are displayed. The histograms show alterations of the intensity and the degree of heterogeneity of the redox states of the organ tissue under study.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.