Activation of cholesterol oxidase for cholesterol assay

US4164448A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Peter Roeschlau · Seeshaupt, DE
• Gunter Lang · Wernau, DE
• Klaus Beaucamp · Tutzing, DE
• Erich Bernt · Munich, DE
• Wolfgang Gruber · Bischofswerda, DE

Key dates

• Filing date: Feb 14, 1977
• Grant date: Aug 14, 1979
• Expiry date: Feb 14, 1997

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/60
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Cholesterol oxidase free of unstabilizing residual traces of detergent is activated by adding to the cholesterol oxidase, before use thereof, at least one surface-active compound with lipophilic and hydrophilic properties. Diagnostic agents for determining cholesterol comprising cholesterol oxidase, a hydrogen peroxide detection system, a buffer, and at least one surface-active compound having lipophilic and hydrophilic properties are provided.