Preparation of albumin using PEG and EDTA
US4164496A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Aug 23, 1978 |
| Grant date | Aug 14, 1979 |
| Priority date | — |
| Expiry date | Aug 23, 1998 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/83
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for the large scale preparation of clinical albumin are disclosed. One method includes dilution of plasma with a NaCl solution containing disodium ethylene dinitrilo tetraacetate and a stabilizer such as sodium caprylate. The resulting solution is then subjected to heating and cooling steps, followed by the addition of polyethylene glycol to precipitate impurities, with albumin remaining in the supernatant. Isoelectric precipitation is then employed to recover the desired albumin product, which is devoid of the albumin dimer. In an alternative method, ethanol is employed as the precipitating agent rather than polyethylene glycol.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.