Method for the determination of substrates or enzyme activities

US4168205A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Josef Danninger · Gräfelfing, DE
• Ulfert Deneke · Fürth, DE
• Gunter Lang · Wernau, DE
• Gerhard Michal · Tutzing, DE
• Peter Roeschlau · Seeshaupt, DE

Key dates

• Filing date: Apr 28, 1977
• Grant date: Sep 18, 1979
• Expiry date: Apr 28, 1997

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/26
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

In a process for the determination of substrates or enzyme activities in a test batch, utilizing a conventional redox reaction as a measurement reaction, the improvement comprising carrying out the process in the presence of ascorbate oxidase; the addition of ascorbate oxidase substantially eliminates the interfering effect of ascorbic acid frequently found in the test sample.