Specific sorbent having D-aminoacid moiety covalently bonded to solid particulate hydrophilic polymeric carrier
US4171412A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 17, 1975 |
| Grant date | Oct 16, 1979 |
| Priority date | — |
| Expiry date | Apr 17, 1995 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01J2220/58
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The invention relates to specific sorbents based on hydrophylic polymeric gels, preferably of a macroporous character, which carry covalently bonded D-aminoacid or peptide which contain D-aminoacid units. The basic carrier prepared by copolymerization of hydroxyalkyl esters or hydroxyalkylamides of acrylic and methacrylic acid with crosslinking acrylate or methacrylate comonomers are modified by the reaction with diamines, aminoacids or dicarboxylic acids and the resulting carboxyterminal or aminoterminal groups are condensed with D-analogs of aminoacids or peptides. The peptide containing D-aminoacids can be also stepwise synthetized on the surface of the carrier. The specific sorbents are further used as insolubilized inhibitors in the affinity chromatography of proteolytic enzymes. They do not undergo an irreversible inactivation during repeated application and are suitable for technological purposes because of their mechanical and hydrolytical stability.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.