Process for the isolation of alkaloid components from the plant Vinca Rosea L.
US4172077A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 22, 1978 |
| Grant date | Oct 23, 1979 |
| Priority date | — |
| Expiry date | May 22, 1998 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07D519/04
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Tumor inhibiting alkaloids are recovered from Vinca Rosea L. by a method which comprises extracting the dried leaves with a solvent selected from the group consisting of an alkanol having 1 to 5 carbon atoms, a mixture of an alkanol having 1 to 5 carbon atoms and a dilute, aqueous solution, benzene and benzene homologs; subsequently purifying the alkaloid extract obtained by a phase-change method between immiscible solvents, precipitating the main amount of dimeric alkaloids in form of their sulphuric acid addition salts, isolating leurosine, vincistine, vinblastine, desacetoxy-vinblastine, N-desmethyl-vinblastine and desacetyl-vinblastine from the salt mixture obtained, and then completing the separation and isolation of the remaining alkaloids by separation and isolation by adjusting the pH-value of the mother liquor, after precipitation and removal of the salt mixture, to 5.5 to 10, extracting the solution with a water-immiscible organic solvent and separating vindoline, catharantine, 3',4' -anhydrovinblastine and leurosine from the extract by chromatography, extracting vindoline in the pH-range of 2.5 to 3.5 and catharantine 3',4'-anhydrovinblastine and leurosine in the pH-rang…
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