Continuous cell culture method and apparatus
US4178209A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 6, 1978 |
| Grant date | Dec 11, 1979 |
| Priority date | — |
| Expiry date | Mar 6, 1998 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12M27/02
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A cell culture system and apparatus is provided for the suspension culturing of mammalian cells in which fresh media can be added and spent medium filtered and withdrawn on a continuous or semi-continuous basis without any substantial cell disruption and clogging of the filter with cell debris. The apparatus comprises a hollow flask assembly having a stationary hollow shaft means and filter unit suspended downwardly from the top thereof, and a rotatable agitator means concentrically disposed about said filter unit, said filter unit being in fluid communication with the top opening of the flask through the interior of said hollow shaft means.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.