Fermentation process for the production of (+)-2-(5.beta.-n-butyl-4.beta.-hydroxy-2-oxo-tetrahydrofuran-3.beta.-yl) -2.alpha.-methylacetic acid
US4178213A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 27, 1977 |
| Grant date | Dec 11, 1979 |
| Priority date | — |
| Expiry date | Dec 27, 1997 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/933
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The invention concerns a fermentation process for the production of (+)-2-(5.beta.-n-butyl-4.beta.-hydroxy-2-oxo-tetrahydrofuran-3.beta.-yl)-2 .alpha.-methylacetic acid, which compound has useful ulcer-healing properties. The process is characterised by cultivating an organism known to produce dihydrocanadensolide, such as Penicillium canadense, under generally conventional conditions and then extracting the culture filtrate at a pH in the range pH 3.0-7.0 with a suitable solvent, such as ethyl acetate, and then evaporating the extract to dryness. In a preferred embodiment the extraction is carried out on the culture filtrate at pH 4.0-4.5.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.