(2S,3R)-3-amino-2-hydroxy-4-phenylbutanoic acid

US4179573A · kind A · utility

Assignee

• Zaidan Hojin Biseibutsu Kagaku Kenkyu Kai · JP

Inventors

• Hamao Umezawa · Tokyo, JP
• Takaaki Aoyagi
• Tomio Takeuchi · Tokyo, JP
• Masa Hamada · Hoya, DE
• Yoshiro Okami · Tokyo, JP

Key dates

• Filing date: Aug 19, 1977
• Grant date: Dec 18, 1979
• Expiry date: Aug 19, 1997

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P13/04
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention provides the amino acid (2S,3R)-3-amino-2-hydroxy-4-phenylbutanoic acid which is prepared by hydrolysis of bestatin. Bestatin is a chemical which inhibits aminopeptidase B, leucine aminopeptidase and bleomycin hydrolase, enhances the antitumor effect of bleomycin, has the chemical name [(2S,3R)-3-amino-2-hydroxy-4-phenylbutanoyl]-L-leucine, has the following structure ##STR1## and is prepared by cultivating a strain of streptomyces which produces bestatin in a nutrient medium under aerobic conditions until substantial activity inhibitory to aminopeptidase B is imparted to said cultured medium and then recovering said bestatin from said cultured medium. A preferred strain is Streptomyces olivoreticuli FERM-P No. 2590 (A.T.C.C. 31159).