Composition and method for the determination of cholesterol

US4181575A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Wolfgang Gruber · Bischofswerda, DE
• Gunter Lang · Wernau, DE
• Michael Nelboeck-Hochstetter
• Peter Roeschlau · Seeshaupt, DE
• Hans Seidel · Tutzing, DE
• Detlef von Hoerschelmann · Wielenbach, DE

Key dates

• Filing date: Oct 13, 1977
• Grant date: Jan 1, 1980
• Expiry date: Oct 13, 1997

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/60
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

In a process for the determination of total or bound cholesterol by liberating any bound cholesterol using cholesterol esterase and simultaneously or subsequently determining the liberated cholesterol and any initially free cholesterol with a cholesterol reacting enzyme, the improvement comprising using as the cholesterol reacting enzyme an NAD or NADP-dependent cholesterol dehydrogenase obtained from an anaerobic microorganism, e.g., Eubacterium sp. ATCC 21408, or from mammalian tissue, e.g., liver.