Polyamide-fixed biologically active protein

US4182695A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Jurgen Horn · Egelsbach, DE
• Winfried Albert · Eberfing, DE
• Hans-Georg Batz · Tutzing, DE
• Michael Nelbock-Hochstetter · Tutzing, DE

Key dates

• Filing date: Feb 16, 1978
• Grant date: Jan 8, 1980
• Expiry date: Feb 16, 1998

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/816
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Biologically active protein compositions comprising protein or a protein substrate bound onto a polyamide of the general formula: ##STR1## in which R and R.sub.1, which may be the same or different, are polyamide residues bound onto the amido group, PA1 R.sub.2 is the residue of a compound which can be condensed with formaldehyde and which contains at least one further reactive group, PA1 R.sub.3 is the residue of a bi- or poly-functional protein reagent, PA1 n is 0 or 1, and PA1 P is a biologically active protein; these compositions are obtainable by the reaction of a polyamides with amounts, equimolar to one another, of formaldehyde and of a compound condensable with formaldehyde, in a solvent for polyamides, reacting the product thereby obtained with a bi- or poly-functional protein reagent and coupling the resulting reaction product with the biologically active protein or protein substrate.