Process for purifying urokinase
US4189350A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 1, 1978 |
| Grant date | Feb 19, 1980 |
| Priority date | — |
| Expiry date | Jun 1, 1998 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y304/21073
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for purifying urokinase by contacting a crude urokinase-containing solution with a strongly acidic cation exchanger having sulfonic acid groups and adjusted to the NH.sub.4.sup.+ type to adsorb urokinase on said cation exchanger and then eluting urokinase from said exchanger, characterized in that urea is previously added to the crude urokinase-containing solution in such a rate that the final molar concentration of urea in the solution is 3 to 8 M. This process is capable of providing purified urokinase having substantially constant and high specific activity from a crude urokinase-containing solution with a high recovery regardless of purity of the crude urokinase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.