Reagent systems, enzymatic assays and compositions therefor
US4189536A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Nov 9, 1977 |
| Grant date | Feb 19, 1980 |
| Priority date | — |
| Expiry date | Nov 9, 1997 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/58
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An improved reagent system for chemical determinations and novel compositions therefor provide improved reagent stability, particularly in a dry, storage stable phase. For determinations in which hexokinase and glucose-6-phosphate dehydrogenase are indicator enzymes, glycine and taurine are combined with bovine serum albumin to provide a bulking agent for the indicator enzymes which also acts as a stabilizer. A novel salt, the tris (hydroxymethyl)aminomethane salt of 2 mercaptosuccinic acid, acts as an activator or stabilizer in chemistries utilizing the indicator enzymes, namely determinations of creatine phosphokinase or glucose. The novel salt also acts as a stabilizer in a urea nitrogen determination. Dry reagent components are bulked in triethanolammonium terephthalate. Novel CPK, glucose and serum urea nitrogen determinations are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.