Enzymatic process for preparing [.gamma.-.sup.32 P]-labeled nucleotides
US4209589A · kind A · utility
2Cited by
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16Claims
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Key dates
| Filing date | Jul 13, 1978 |
| Grant date | Jun 24, 1980 |
| Priority date | — |
| Expiry date | Jul 13, 1998 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P19/32
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
ATP and other nucleoside triphosphates labeled in the .gamma.-phosphate with .sup.32 P are prepared from L-.alpha.-glycerophosphate and their corresponding nucleoside diphosphates by a series of enzymatic reactions in the presence of NAD.sup.+, preferably regenerated by lactate dehydrogenase and pyruvate. The resulting [.gamma.-.sup.32 P]nucleotides are useful as reagents for analytical determinations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.