Reagent and method for the determination of cholesterol

US4212938A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Wolfgang Gruber · Bischofswerda, DE
• Hans U. Bergmeyer
• Erich Bernt · Munich, DE
• Alexander Hagen · Tutzing, DE
• Peter Roeschlau · Seeshaupt, DE
• Gunter Lang · Wernau, DE
• Klaus Beaucamp · Tutzing, DE

Key dates

• Filing date: May 15, 1973
• Grant date: Jul 15, 1980
• Expiry date: May 15, 1993

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/60
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Cholesterol is determined by incubating a sample suspected of containing cholesterol in an aqueous medium with cholesterol oxidase and determining either the oxygen consumption, the hydrogen peroxide formed, or cholestenone, as a measure of the initial cholesterol content; reagent compositions comprising cholesterol oxidase and a system for the determination of H.sub.2 O.sub.2 or for the determination of cholestenone are provided.