Assay of serine proteases using novel chromogenic enzyme substrates

US4214049A · kind A · utility

Assignee

• AB Kabi · SE

Inventors

• Bo T. af Ekenstam
• Leif E. Aurell · Särö, SE
• Karl G. Claeson
• Birgitta Karlsson · Timrå, SE
• Stig I. Gustavsson · Mölndal, SE
• Gun A. Olausson · Mölndal, SE

Key dates

• Filing date: Oct 27, 1978
• Grant date: Jul 22, 1980
• Expiry date: Oct 27, 1998

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2337/12
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Method for the quantitative determination of serine proteases which includes contacting the substance for which the determination is desired with a diagnostically active chromogenic substrate with good stability and high specificity to serine proteases having the formula: H--D--A.sub.1 --A.sub.2 --A.sub.3 --NH--R or salts thereof, wherein A.sub.1 is Gly, Ala, Val, Leu, Ile, Pip, Pro, or Aze; A.sub.2 is Gly, Ala, Val, Leu, Ile, Pip, Pro, Aze, or Phe; A.sub.3 is Arg, Lys, or Orn, and R is a chromophoric group.