Precipitating reagent and method for isolation and determination of high density lipoproteins in human serum
US4215993A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Dec 4, 1978 |
| Grant date | Aug 5, 1980 |
| Priority date | — |
| Expiry date | Dec 4, 1998 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/044
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A precipitating reagent and method is provided for isolating high density lipoproteins from low density lipoproteins in human serum together with a quantitative determination of high density lipoprotein cholesterol. Precipitation of low density lipoproteins is accomplished by the precipitating reagent without the addition of metal ions into the sample. The precipitating reagent lowers the pH of the human serum approximately to the isoelectric point of the low density lipoproteins through the use of an organic buffer. The precipitating reagent also contains a polyanion and neutral polymer. The preferred composition of the precipitating reagent contains about 0.4% phosphotungstic acid by weight thereof, about 2.5% of polyethylene glycol by weight thereof and 2-(N-morpholino) ethane sulfonic acid as the buffer present in a concentration of from about 0.2 molar to about 0.5 molar. According to the method provided, the precipitating reagent is added to the human serum sample thereby causing the low density lipoproteins to form a precipitate, leaving the high density lipoproteins in the resulting supernatant liquid. The supernatant is separated from the precipitate and a cholesterol assa…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.