Dipeptide chromogenic substrates
US4217269A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Mar 23, 1979 |
| Grant date | Aug 12, 1980 |
| Priority date | — |
| Expiry date | Mar 23, 1999 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/802
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention comprises compounds of the formula ##STR1## and the biologically acceptable acid addition salts thereof, wherein R.sub.1 represents an alkylene having 2-4 carbon atoms; R.sub.2 is selected from the group consisting of amino and quanyl; R.sub.3 is selected from the group consisting of nitrophenyl, methylnitrophenyl, dinitrophenyl, naphthyl and nitronaphthyl; X represents halo or lower alkyl having 1-4 carbon atoms; and n is 3 or 4. The compounds of the present invention are analytical reagents useful for measuring proteolytic enzymes such as thrombin and trypsin. The enzymatic hydrolysis of the invention compounds provides a chromogenic amine by which the proteolytic enzyme concentration can be determined spectrophotometrically.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.