Assay for proteins and polypeptides
US4219337A · kind A · utility
18Cited by
2References
6Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 27, 1978 |
| Grant date | Aug 26, 1980 |
| Priority date | — |
| Expiry date | Apr 27, 1998 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6839
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An assay for proteins or polypeptides in solution using a reagent of Coomassie Brilliant Blue G250 dye in perchloric or hydrochloric acid. Upon mixture of the acidic reagent with a protein sample, the dye couples with the protein and undergoes a color change, following which the absorbance of the mixture can be measured to provide a quantitative analysis for the protein in the sample; the same procedure may be used for sample solutions containing polypeptides. Proteins and polypeptides with molecular weights above about 2000-3000 may be assayed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.