Patent · US Expired

Immunochemical conjugates: method and composition

US4220565A · kind A · utility

31Cited by
1References
4Claims
0Family size

Assignee

Inventor

Key dates

Filing dateJan 18, 1979
Grant dateSep 2, 1980
Priority date—
Expiry dateJan 18, 1999

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S530/806
  • WIPO fieldPharmaceuticals
  • WIPO sectorChemistry

Abstract

Techniques for preparing stable conjugates of protein-D-GL which can then be isolated in pure form are disclosed, using hen egg ovalbumin (OVA) as a prototype protein, and conjugation and purification methods are described which involve (1) application of a coupling method employing m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS) as the coupling reagent, and (2) introduction of biotin moieties into D-GL molecules to allow application of avidinbiotin system for affinity chromatographic purification of conjugates. These methods are applicable to the preparation of D-GL conjugates of insulin and Ragweed Antigen E and are of general applicability.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.