Method and apparatus for detecting biological particles by fluorescent stain
US4222743A · kind A · utility
Inventor
Key dates
| Filing date | Jul 20, 1978 |
| Grant date | Sep 16, 1980 |
| Priority date | — |
| Expiry date | Jul 20, 1998 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/818
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A metal-covered substrate is first adsorbed with a layer of known biological particles. This active substrate is then put into a solution containing an unknown amount of second biological particles that will bind the first kind of particles which are randomly distributed in the layer. A third biological particle bound to a fluorescent dye is applied to this substrate which is then placed in an apparatus having a light source inducing fluorescent emission, and a photon counting system to measure the amount of fluorescent photon. The metal-covered substrate reflects the exciting photon toward a photon trap so that few exciting photons will go to the photon counting system, while the induced signal is reflected toward the photon counting system and more induced signal will be measured.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.