Affinity chromatography of Vibrio cholerae enterotoxin-ganglioside polysaccharide and the biological effects of ganglioside-containing soluble polymers
US4225487A · kind A · utility
Inventors
Key dates
| Filing date | Apr 13, 1978 |
| Grant date | Sep 30, 1980 |
| Priority date | — |
| Expiry date | Apr 13, 1998 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S930/29
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
Columns of polysaccharide, e.g., agarose, derivatives comprising covalently bonded gangliosides quantitatively adsorb the .sup.125 I-labeled cholera toxin of chromatographed samples. The most preferred derivatives are those wherein the gangliosides are coupled to "macromolecules" [native albumin, denatured albumin, poly(L-lysine) and poly(L-lysyl-DL-alanine) graft copolymers] which themselves are covalently bonded to the, e.g., agarose. The soluble ganglioside polymers can prevent the binding of .sup.125 I-labeled toxin to liver membranes as well as block completely the lipolytic activity of cholera toxin on fat cells, and thus are useful in the management of the manifestations of clinical cholera.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.