Tris (hydroxymethyl) aminomethane salt of 2-mercaptosuccinic acid
US4229369A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Apr 25, 1979 |
| Grant date | Oct 21, 1980 |
| Priority date | — |
| Expiry date | Apr 25, 1999 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/54
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An improved reagent system for chemical determinations and novel compositions therefor provide improved reagent stability, particularly in a dry, storage stable phase. For determinations in which hexokinase and glucose-6-phosphate dehydrogenase are indicator enzymes, glycine and taurine are combined with bovine serum albumin to provide a bulking agent for the indicator enzymes which also acts as a stabilizer. A novel salt, the tris (hydroxymethyl)aminomethane salt of 2 mercaptosuccinic acid, acts as an activator or stabilizer in chemistries utilizing the indicator enzymes, namely determinations of creatine phosphokinase or glucose. The novel salt also acts as a stabilizer in a urea nitrogen determination. Dry reagent components are bulked in triethanolammonium terepthalate. Novel CPK, glucose and serum urea nitogen determinations are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.