Process and reagent for the kinetic determination of enzyme substrates

US4229527A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Joachim Ziegenhorn · Starnberg, DE
• August W. Wahlefeld · Hohenpeißenberg, DE
• Alexander Hagen · Tutzing, DE
• Wolfgang Gruaber · Tutzing, DE
• Hans U. Bergmeyer

Key dates

• Filing date: Oct 23, 1978
• Grant date: Oct 21, 1980
• Expiry date: Oct 23, 1998

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/81
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Kinetic determination of enzyme substrates by means of coupled reactions comprising selecting the reaction parameters so that the most specific part reaction of a reaction sequence becomes rate-determining for the whole reaction sequence wherein the rate-determining part reaction follows first or pseudo-first order kinetics.