Method of magnetic separation of cells and the like, and microspheres for use therein
US4230685A · kind A · utility
185Cited by
2References
10Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 28, 1979 |
| Grant date | Oct 28, 1980 |
| Priority date | — |
| Expiry date | Feb 28, 1999 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/828
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Magneticaly-responsive microspheres having Protein A associated with the outer surfaces thereof are reacted with antibodies selective to the cells, bacteria, or viruses to be separated from a mixed population to attach the antibodies in oriented relation with their Fab arms extending outwardly, and the microspheres are then used in a magnetic separation procedure. The preferred microspheres are prepared from a mixture of albumin, Protein A, and magnetic particles, the microspheres being prepared so that the Protein A is present in the exterior surfaces for antibody binding.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.