Preparation of water-insoluble enzyme compositions

US4230803A · kind A · utility

Assignee

• Kali-Chemie Aktiengesellschaft · DE

Inventors

• Guenter Weidenbach · Hannover, DE
• Dirk Bonse · Lehrte, DE

Key dates

• Filing date: May 31, 1978
• Grant date: Oct 28, 1980
• Expiry date: May 31, 1998

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N11/14
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Enzymes are covalently bonded to porous inorganic supports by a process wherein maximum activity of enzyme in the water-insoluble enzyme composition is achieved with the lowest possible amount of enzyme. The process involves selecting an inorganic carrier having a most frequent pore-diameter that results in highest enzymatic activity possible per weight unit of the insoluble enzyme composition at the given enzyme concentration within the insoluble enzyme composition, and contacting the selected inorganic carrier with an enzyme solution containing an amount of enzyme which is sufficiently low so that the enzyme in the insoluble enzyme composition has a specific activity which is substantially the same as that of the enzyme in solution in the free state.