Competitive enzyme-linked immunoassay
US4235960A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 29, 1977 |
| Grant date | Nov 25, 1980 |
| Priority date | — |
| Expiry date | Jul 29, 1997 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A competitive enzyme-linked immunoassay for a soluble analyte in which the analyte in the unknown sample and the same analyte attached to a solid phase (immunosorbent) compete for a selected quantity of a soluble first antibody to the substance; a second or bridging antibody is then linked to any first antibody linked to the immunosorbent as a result of the competitive reaction, following which a soluble enzyme/antibody immune complex is linked to bridging antibody on the immunosorbent. The enzymatic activity associated with the thusly-formed enzyme-immunosorbent complex or with the non-immunosorbent fraction of enzyme is measured to determine the presence and/or concentration of analyte in the unknown sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.