Macroporous polymeric carrier for covalently binding proteins, its preparation and its use for fixing active proteins

US4235973A · kind A · utility

Assignee

• BASF SE

Inventors

• Chung-Ji Tschang · Bad Dürkheim, DE
• Heinrich Klefenz · Hochdorf-Assenheim, DE
• Axel Sanner · Bischofswerda, DE

Key dates

• Filing date: Jul 12, 1979
• Grant date: Nov 25, 1980
• Expiry date: Jul 12, 1999

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C08F8/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A macroporous crosslinked sytrene resin, used as a carrier for covalently binding proteins, which resin contains isocyanate, thioisocyanate or aldehyde groups as protein-binding groups and may or may not contain sulfonic acid groups--which may also be in the form of the sodium salt or of sulfonic acid amide groups--as hydrophilic groups. The carrier according to the invention is prepared from a sulfochlorinated macroporous crosslinked styrene resin by reacting the sulfonic acid chloride groups with an .alpha.,.omega.-diamino compound, with hydrazine or with an .alpha.,.omega.-diaminodiether, converting any sulfonic acid chloride groups which may still be present to free sulfonic acid groups, their sodium salt or a sulfonamide group, and then reacting the terminal amino groups with phosgene, thiophosgene or a diisocyanate in order to produce the binding groups, which in turn fix a biologically active protein by covalent bonds.