Antibiotic detection method
US4239852A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jun 12, 1978 |
| Grant date | Dec 16, 1980 |
| Priority date | — |
| Expiry date | Jun 12, 1998 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/18
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A process for rapidly detecting as little as 0.001 I.U. ml of antibiotic in a sample of liquid such as milk. The process comprises the steps of incubating the sample together with a tagged antibiotic or antibiotic precursor and antibiotic sensitive cells under conditions which allow antibiotic molecules to attach to receptor sites in or on the cells, separating the cells with immobilized antibiotic from the remainder of the reaction mixture, and determining the quantity of tagged antibiotic on the cells. The amount of tagged antibiotic on the cells is a function of the quantity of antibiotic present in the sample. The process is well suited for detecting penicillin-type antibiotics and may be practiced using peroxidase-tagged 6-amino penicillanic acid and sonicated, penicillin supersensitive Bacillus stearothermophilus immobilized on a support.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.