Assay for reserve bilirubin binding capacity
US4240797A · kind A · utility
13Cited by
5References
9Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Oct 18, 1977 |
| Grant date | Dec 23, 1980 |
| Priority date | — |
| Expiry date | Oct 18, 1997 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/24
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Spin labels, especially dianionic aromatic spin labels which can bind to the first specific high-affinity bilirubin-binding site on serum albumin, and which are quantitatively displaceable into solution from said site in the presence of bilirubin, can be used in an assay for bilirubin-binding capacity. In the assay, an excess of spin label is mixed with the serum albumin or serum and is titrated with standard bilirubin. ESR spectroscopy indicates a change in the rate of spin label release with addition of bilirubin, giving a value indicative of bilirubin-binding capacity. Novel dianionic aromatic spin labels are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.