Single-incubation two-site immunoassay
US4244940A · kind A · utility
63Cited by
6References
20Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Sep 5, 1978 |
| Grant date | Jan 13, 1981 |
| Priority date | — |
| Expiry date | Sep 5, 1998 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/808
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This invention provides a two-site immunoassay method for a ligand utilizing a single incubation. The multivalent ligand, labeled receptor for the ligand and unlabeled receptor for the ligand covalently bound to a solid-phase support are incubated as a substantially stable suspension to produce a solid and liquid phase. The solid and liquid phases are separated from each other and the labeled receptor in either phase is quantitated. The method may be used to assay for human thyroid stimulating hormone using purified, radioactively labeled antibodies and unlabeled antibodies covalently bound to hydrolyzed polyacrylamide particles.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.