Protein immobilization on chelating resins
US4246350A · kind A · utility
2Cited by
5References
7Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 1, 1979 |
| Grant date | Jan 20, 1981 |
| Priority date | — |
| Expiry date | Mar 1, 1999 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/816
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Proteins such as enzymes are immobilized on macroporous resins having recurring bis-picolylamine, imino diacetate or hydroxyethyl picolylamine chelating sites. Immobilization is carried out by contacting the resin with a multivalent metal ion selected from the transition metals or rare earth metals and adsorbing the protein to the resin. This immobilization technique permits strongly binding protein to a high surface area resin. The protein can be readily removed from the resin by simply flushing the resin with an appropriate solution.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.