Enzyme immobilization with pullulan gel
US4247642A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 16, 1978 |
| Grant date | Jan 27, 1981 |
| Priority date | — |
| Expiry date | Feb 16, 1998 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N11/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Enzymes are immobilized by a process wherein the carrier used for immobilizing is a hydrophilic pullulan gel having a three-dimensionally reticulated structure obtained by the reaction between pullulan and a bifunctional compound capable of forming an ether linkage with the hydroxyl group contained in the glucose unit of pullulan, or an ionic pullulan gel obtained by the reaction between said hydrophilic pullulan gel and a compound having at one end an ionizable group and at the other end a functional group capable of forming an ether linkage with the hydroxyl group in the pullulan gel in the presence of an alkaline coumpound. Immobilized enzymes prepared according to this process have a high activity and good retention of the activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.