Patent · US Expired

Process for the microbial production of alcoholoxidase

US4250261A · kind A · utility

10Cited by

1References

5Claims

0Family size

Assignee

Kernforschungsanlage Julich Gesellschaft m.b.H.

Inventors

Lothar Eggeling · Jülich, DE
Manfred Paschke · Jülich, DE
Hermann Sahm · Jülich, DE

Key dates

Filing date	Jul 9, 1979
Grant date	Feb 10, 1981
Priority date	—
Expiry date	Jul 9, 1999

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY10S435/93
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A method of producing alcohol oxidase wherein Hansenula polymorpha yeast is cultivated in a nutrient broth containing inorganic nutrient salts of which either the phosphate, nitrate or potassium is present in a cell-growth-limiting concentration at a temperature of 25.degree. to 45.degree. C. and a pH of 4.0 to 6.0 with oxygen being supplied as air for a mixture of air and oxygen. According to the invention, an organic substrate consisting of glycerin, sorbite or xylose, noncatabolite-repressive to alcohol-oxidase is added to the broth in a concentration of 0.1 to 1.0% in a nutrient solution at a rate of 0.03 to 0.15 .sup.-1.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.