Chromogenic enzyme substrates

US4252715A · kind A · utility

Assignee

• AB Kabi · SE

Inventors

• Leif E. Aurell · Särö, SE
• Karl G. Claeson

Key dates

• Filing date: Jul 2, 1979
• Grant date: Feb 24, 1981
• Expiry date: Jul 2, 1999

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S930/28
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

New chromogenic substrates suitable for serine proteases, specially suitable for diagnostic determination of factor Xa, which are represented by the following formula: EQU R.sub.1 -A.sub.1 -A.sub.2 -Gly-Arg-NH-R.sub.2 or its salts, where R.sub.1 is selected from the group of hydrogen, alkanoyl having from 1 to 12 carbon atoms, cyclohexylcarbonyl, benzoyl, benzoyl substituted with one or two halogen atoms, methylamine or phenyl groups, benzene sulphonyl and toluenesulphonyl; R.sub.2 is a chromophoric group; A.sub.1 is selected from the group of a single bond, and the amino acids selected from the group of Gly, Ala, Val, Leu, Ileu, Pro, Met, Phe and Tyr; and A.sub.2 is selected from the group of the amino acids Glu, Gln, Asp, and Asn.